Biochemistry of proteins IS-FCEE-00135

Description:
Basic equipment in biochemical laboratory. Proteins as colloids. Protein solubility and protein excretion. Protein denaturation. Protein content in various biological samples. Structural and metabolic proteins in Eucaryota and Procaryota cells. Quantitative quantification of the protein content by using Lowry method, the ultraviolet absorbance method and the Bradford method in various biological samples. Electrophoretic separation of proteins from various biological samples by vertical polyacrylamide gel electrophoresis (SDS-PAGE method). Isolation of ribonucleoproteins from bovine (or pancreas).

Laboratories

1.\tHealth and Safety Rules. Introduction to the organization of biochemical laboratory. Preparation of hydrophilic colloid solutions. Examination of the solubility of globulins in water and diluted saline solutions. Protein thermal denaturation and protein coagulation.
2.\tIsolation of total protein from different types of biological samples: in vitro culture of human cells, bacterial cells and sewage sludge. Quantitative determination of protein in biological samples by using Lowry method.
3.\tDetermination of protein content in biological samples by ultraviolet absorbance measurement. Determination of protein content in biological samples by using Bradford's method.
4.\tElectrophoretic separation of proteins from various biological samples by vertical polyacrylamide gel electrophoresis
5.\tExtraction of ribonucleoproteins from fresh thymus or pancreatic bovine tissue.

Requirements:
Knowledge
1.\tStudent understands hygiene and safety rules during work in biochemical laboratory
2.\tStudent knows and understand biochemical and molecular basis of cell functioning.
3.\tStudent possess a knowledge concerning advanced laboratory, measuring and imaging techniques which are used for research and biotechnological purposes.
4.\tStudent knows a variety of techniques and research tools which are used in biochemical laboratory.
5.\tStudent is able to collect an empirical data and interpret them.

Skills
1.\tStudent learned the basic biochemical techniques.
2.\tStudent has the ability to work with laboratory equipment.
3.\tStudent knows the use of biochemical research methods in modern biology and biotechnology.
4.\tStudent can carried out simple research experiments and analyses under the supervisor guidance.
5.\tStudent is able to conduct a proper reasoning according to scientific data.
6.\tStudent knows how to work in a team, carrying out research projects using biochemical techniques

Social competences
1.\tDemonstrates ability of team working.
2.\tDemonstrates responsibility for his own work and entrusted equipment displays respect to his own work and the work of others.
3.\tUnderstands a need of transfer of new achievements in biotechnology to society and is able to transfer it in understandable manner.