Human Cell and Tissue Culture IS-FCEE-00122

Description:
Basic equipment in cell culture laboratory. Biosafety levels in cell culture laboratory. Aseptic techniques and types of biological contamination. Characteristic of the cell culture environment with special regards to media, pH, CO2 and temperature. Basic chemical reagents used for cell culture: media and additives to the media. Basic methods in cultured cells maintaining: subculturing adherent and suspension cells, freezing and thawing cells and counting cells in hemocytometer. Selecting an appropriate cell line. Morphological types of cell lines. Cultures and cell line types (primary cultures of cells, the culture of suspension cells, the cell lines, pure cell lines, clonal cell lines, mixed cultures, the culture cells in suspension).

Laboratories

1.tHealth and Safety Rules. Introduction to the organization of cell and tissue culture laboratory.
Preparation of the materials and solutions for cell culture. Sterilization.
Workplace preparation. Media change in the adherent culture.

2.tSubculturing of the MCF-7/fibroblast adherent and MOLT-4 suspension culture.

3.tVarious methods for cell counting and basic principles for preparing MCF-7 and fibroblasts adherent cells for the experiment.

4.tFreezing and thawing of adherent and suspension cells. The creation of cell banks.

5.tVarious methods in estimation of cells viability. Cytotoxicity tests as a basic tests in preclinical studies.

Requirements:
Knowledge
1.tStudent understands hygiene and safety rules during work with human cell lines
2.tStudent knows and understand biochemical and molecular basis of cell functioning.
3.tStudent possess a knowledge concerning advanced laboratory, measuring and imaging techniques which are used for research and biotechnological purposes.
4.tStudent knows a variety of techniques and research tools which are used in cell and tissue culture laboratory.
5.tStudent is able to collect an empirical data and interpret them.

Skills
1.tStudent learned the basic cell culture techniques.
2.tStudent has the ability to work with an inverted light microscope.
3.tStudent knows the use of cells and tissues research methods in modern biology and biotechnology.
4.tStudent can carried out simple research experiments and analyses under the supervisor guidance.
5.tStudent is able to conduct a proper reasoning according to scientific data.
6.tStudent knows how to work in a team, carrying out research projects using tissue or cell cultures.

Social competences
1.tDemonstrates ability of team working.
2.tDemonstrates responsibility for his own work and entrusted equipment displays respect to his own work and the work of others.
3.tUnderstands a need of transfer of new achievements in biotechnology to society and is able to transfer it in understandable manner.